5HSH

Crystal structure of the G291R mutant of human phosphoglucomutase 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2015-10-25
Detector	RDI CMOS_8M
Wavelength(s)	1.00003
Spacegroup name	P 41 21 2
Unit cell lengths	170.780, 170.780, 99.287
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.537 - 2.650
R-factor	0.2321
Rwork	0.230
R-free	0.27330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5epc
RMSD bond length	0.010
RMSD bond angle	1.214
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.537	2.750
High resolution limit [Å]	2.650	2.650
Rmerge	0.107	2.810
Number of reflections	48369
<I/σ(I)>	18	0.9
Completeness [%]	100.0	100
Redundancy	14	13.1
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.7	292	~1.0M Sodium Citrate, 0.1M CHES Buffer pH 9.7.