5HS7
Reduced form of the transcriptional regulator YodB from B. subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-24 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.780, 50.798, 50.354 |
| Unit cell angles | 90.00, 95.49, 90.00 |
Refinement procedure
| Resolution | 50.120 - 1.700 |
| R-factor | 0.19818 |
| Rwork | 0.197 |
| R-free | 0.22077 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hqm |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.539 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.120 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.056 | 0.451 |
| Number of reflections | 22351 | |
| <I/σ(I)> | 44.5 | |
| Completeness [%] | 99.0 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2M sodium bromide and 20%(w/v) PEG 3,350 |
