5HS4

Plasmdoium Vivax Lactate dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9796
Spacegroup name	I 2 2 2
Unit cell lengths	75.792, 80.613, 93.377
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.765 - 1.339
R-factor	0.151
Rwork	0.150
R-free	0.17140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a92
RMSD bond length	0.008
RMSD bond angle	1.163
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	1.360
High resolution limit [Å]	1.339	3.630	1.340
Rmerge	0.076	0.051	0.274
Number of reflections	63845
<I/σ(I)>	19.4
Completeness [%]	99.2	97.1	97.3
Redundancy	11.8	13.5	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	ammonium nitrate, PEG-3350, magnesium ion, potassium chloride