5HRU
Crystal structure of Plasmodium vivax LDH in complex with a DNA aptamer called pL1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9796 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 84.477, 84.477, 209.867 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.960 - 1.710 |
Rwork | 0.160 |
R-free | 0.18450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a92 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9-1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.000 | 25.000 | 1.740 |
High resolution limit [Å] | 1.710 | 4.630 | 1.710 |
Rmerge | 0.103 | 0.073 | 0.292 |
Number of reflections | 82531 | ||
<I/σ(I)> | 15 | ||
Completeness [%] | 99.8 | 99.3 | 99.9 |
Redundancy | 21.4 | 26.3 | 15.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.19 | PEG-20K, NDSB-195, magnesium chloride |