5HRG
The crystal structure of AsfvPolX(D51N mutant):DNA4 binary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.060, 51.170, 55.540 |
| Unit cell angles | 68.35, 86.25, 88.20 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.19319 |
| Rwork | 0.191 |
| R-free | 0.23893 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hr9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.085 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 27077 |
| <I/σ(I)> | 6.8 |
| Completeness [%] | 93.8 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100mM HEPES pH 7.5, 20% (w/v) PEG 8000, 200mM Ammonium sulfate 10% (v/v) 2-Propanol |
