5HRD
The crystal structure of AsfvPolX:DNA2 binary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.196, 87.485, 87.914 |
| Unit cell angles | 90.00, 91.45, 90.00 |
Refinement procedure
| Resolution | 29.310 - 1.800 |
| R-factor | 0.1989 |
| Rwork | 0.196 |
| R-free | 0.24456 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hr9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.315 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 75045 |
| <I/σ(I)> | 20.5 |
| Completeness [%] | 93.5 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1M Succinic acid PH 7.0, 15% PGE 3350 |
