5HRC
Crystal structure of an aspartate/glutamate racemase in complex with L-aspartate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.989 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.779, 48.234, 114.784 |
Unit cell angles | 90.00, 95.12, 90.00 |
Refinement procedure
Resolution | 32.730 - 1.765 |
R-factor | 0.2202 |
Rwork | 0.218 |
R-free | 0.25770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hqt |
RMSD bond length | 0.007 |
RMSD bond angle | 0.874 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2257: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.730 | 1.828 |
High resolution limit [Å] | 1.765 | 1.765 |
Number of reflections | 48432 | |
<I/σ(I)> | 20.06 | |
Completeness [%] | 91.0 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 9.5 | 293 | 1.0 M sodium citrate, 0.1 M CHES, 3.0% D (+)-Sucrose |