5HRA
Crystal structure of an aspartate/glutamate racemase in complex with D-aspartate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.989 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.938, 48.248, 116.289 |
Unit cell angles | 90.00, 92.92, 90.00 |
Refinement procedure
Resolution | 32.610 - 1.597 |
R-factor | 0.2337 |
Rwork | 0.233 |
R-free | 0.24980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hqt |
RMSD bond length | 0.009 |
RMSD bond angle | 1.294 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10pre_2090: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.610 | 1.654 |
High resolution limit [Å] | 1.597 | 1.597 |
Number of reflections | 66927 | |
<I/σ(I)> | 16.12 | |
Completeness [%] | 96.0 | |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 9.5 | 293 | 1.0 M sodium citrate, 0.1 M CHES, 3.0% D (+)-Sucrose |