5HR4
Structure of Type IIL restriction-modification enzyme MmeI in complex with DNA has implications for engineering of new specificities
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 61.877, 95.292, 161.969 |
Unit cell angles | 72.84, 89.15, 71.62 |
Refinement procedure
Resolution | 43.047 - 2.596 |
R-factor | 0.2156 |
Rwork | 0.215 |
R-free | 0.24190 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.060 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((dev_2264: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.596 | 2.596 |
Rmerge | 0.128 | 0.511 |
Number of reflections | 91018 | |
<I/σ(I)> | 11.64 | 2.023 |
Completeness [%] | 89.3 | 75.2 |
Redundancy | 3.5 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 10%(w/v) polyethylene glycol (PEG) 8K,0.1M HEPES pH 7.5, 0.2Mcalcium acetate |