5HPN
A circularly permuted PduA forming an icosahedral cage
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97170 |
Spacegroup name | P 42 3 2 |
Unit cell lengths | 144.500, 144.500, 144.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 83.427 - 2.509 |
R-factor | 0.2164 |
Rwork | 0.212 |
R-free | 0.25560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ppd |
RMSD bond length | 0.010 |
RMSD bond angle | 1.352 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.430 | 2.598 |
High resolution limit [Å] | 2.509 | 2.509 |
Number of reflections | 18264 | |
<I/σ(I)> | 14.71 | |
Completeness [%] | 100.0 | |
Redundancy | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 1.8M Ammonium Sulfate 0.1M Tris pH8.5 1.25% w/v Peg 10000 |