5HPG

STRUCTURE AND LIGAND DETERMINANTS OF THE RECOMBINANT KRINGLE 5 DOMAIN OF HUMAN PLASMINOGEN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	123
Detector technology	IMAGE PLATE
Collection date	1997-04
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 2
Unit cell lengths	77.430, 79.200, 30.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 1.660
R-factor	0.166 *
Rwork	0.166
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pkr
RMSD bond length	0.016
RMSD bond angle	0.034
Data reduction software	RIGAKU (R-AXIS)
Data scaling software	R-AXIS ((RIGAKU))
Phasing software	AMoRE
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.260	1.860
High resolution limit [Å]	1.660	1.660 *
Rmerge	0.042	0.087
Total number of observations	42366 *
Number of reflections	16720
<I/σ(I)>	14	3.6
Completeness [%]	72.0	37
Redundancy	2.5	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	drop	PEG8000	12 (%(w/v))
3	1	drop	sodium HEPES	0.05 (M)
4	1	drop		0.075 (M)
5	1	reservoir	PEG8000	24 (%(w/v))
6	1	reservoir	sodium HEPES	0.1 (M)
7	1	reservoir		0.15 (M)