5HPG
STRUCTURE AND LIGAND DETERMINANTS OF THE RECOMBINANT KRINGLE 5 DOMAIN OF HUMAN PLASMINOGEN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.430, 79.200, 30.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 1.660 |
| R-factor | 0.166 * |
| Rwork | 0.166 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pkr |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.034 |
| Data reduction software | RIGAKU (R-AXIS) |
| Data scaling software | R-AXIS ((RIGAKU)) |
| Phasing software | AMoRE |
| Refinement software | PROFFT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.260 | 1.860 |
| High resolution limit [Å] | 1.660 | 1.660 * |
| Rmerge | 0.042 | 0.087 |
| Total number of observations | 42366 * | |
| Number of reflections | 16720 | |
| <I/σ(I)> | 14 | 3.6 |
| Completeness [%] | 72.0 | 37 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 12 (%(w/v)) | |
| 3 | 1 | drop | sodium HEPES | 0.05 (M) | |
| 4 | 1 | drop | 0.075 (M) | ||
| 5 | 1 | reservoir | PEG8000 | 24 (%(w/v)) | |
| 6 | 1 | reservoir | sodium HEPES | 0.1 (M) | |
| 7 | 1 | reservoir | 0.15 (M) |
