5HPF
Crystal Structure of the Double Mutant of PobR Transcription Factor Inducer Binding Domain from Acinetobacter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97934 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 111.348, 111.348, 282.174 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.375 - 2.309 |
| R-factor | 0.1724 |
| Rwork | 0.170 |
| R-free | 0.21520 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.925 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10pre_2104: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.121 | 0.849 |
| Number of reflections | 37742 | |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 99.8 | 96.9 |
| Redundancy | 5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 0.1 M sodium/potassium phosphate pH 5.6 |
