5HPF
Crystal Structure of the Double Mutant of PobR Transcription Factor Inducer Binding Domain from Acinetobacter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97934 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 111.348, 111.348, 282.174 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.375 - 2.309 |
R-factor | 0.1724 |
Rwork | 0.170 |
R-free | 0.21520 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.925 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10pre_2104: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.121 | 0.849 |
Number of reflections | 37742 | |
<I/σ(I)> | 13.2 | |
Completeness [%] | 99.8 | 96.9 |
Redundancy | 5 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 0.1 M sodium/potassium phosphate pH 5.6 |