5HP7
Crystal structures of RidA in the apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Agilent SuperNova |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-17 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 |
| Unit cell lengths | 76.907, 76.907, 40.298 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.670 - 2.000 |
| R-factor | 0.15534 |
| Rwork | 0.153 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b33 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.456 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.700 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.045 | 0.183 |
| Number of reflections | 6001 | |
| <I/σ(I)> | 16.2 | 5.5 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 18% PEG 3350, 0.1 M MES pH6.0 |
