5HOP
1.65 Angstrom resolution crystal structure of lmo0182 (residues 1-245) from Listeria monocytogenes EGD-e
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-13 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 43 |
Unit cell lengths | 110.141, 110.141, 45.028 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.450 - 1.650 |
R-factor | 0.16404 |
Rwork | 0.162 |
R-free | 0.19449 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.548 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.087 | 0.641 |
Number of reflections | 65420 | |
<I/σ(I)> | 44.2 | 3.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.3 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | protein: 15.2 mg/ml in 10 mM Tris-HCl pH 8.3 0.5 mM TCEP crystallization: The PACT Suite C2(#26): 0.1 M PCB buffer pH 5.0 25% (w/v) PEG1500 cryo: crystallization condition |