5HON
Structure of Domain 4 of AbnA, a GH43 extracellular arabinanase from Geobacillus stearothermophilus, in complex with arabinotriose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-X |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-06-17 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.408, 77.150, 68.622 |
Unit cell angles | 90.00, 106.68, 90.00 |
Refinement procedure
Resolution | 37.749 - 2.001 |
R-factor | 0.1663 |
Rwork | 0.164 |
R-free | 0.19690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ho0 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.084 | 0.273 |
Number of reflections | 24952 | |
<I/σ(I)> | 11.4 | 3.7 |
Completeness [%] | 93.6 | 65.4 |
Redundancy | 4.3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 22-24.5% PEG 3350, 0.1M Bis-Tris pH 5.5-6 |