5HO5
MamB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.932 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.165, 96.663, 96.913 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.440 - 1.990 |
| R-factor | 0.20923 |
| Rwork | 0.207 |
| R-free | 0.24898 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.904 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 68.440 |
| High resolution limit [Å] | 1.990 |
| Rmerge | 0.086 |
| Number of reflections | 24550 |
| <I/σ(I)> | 21.7 |
| Completeness [%] | 93.3 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 40% 4-Butyrolactone, 22.5% PEG 3350, 0.1M Mg formate |
