5HO3
MamB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.932 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 97.364, 37.600, 49.652 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.650 - 2.150 |
| R-factor | 0.2046 |
| Rwork | 0.200 |
| R-free | 0.24548 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.647 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.650 | |
| High resolution limit [Å] | 2.150 | |
| Rmerge | 0.048 | 0.449 |
| Number of reflections | 10470 | |
| <I/σ(I)> | 38.03 | |
| Completeness [%] | 99.2 | |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293.15 | 0.1 M HEPES pH 7.5, 25% PEG 3350, 0.2 M LiSO4 |
