5HO2
Crystal structure of AbnA (open conformation), a GH43 extracellular arabinanase from Geobacillus stearothermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.693, 87.541, 129.234 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.430 - 2.370 |
R-factor | 0.16853 |
Rwork | 0.165 |
R-free | 0.24159 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ho0 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.603 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.400 |
High resolution limit [Å] | 2.360 | 2.360 |
Rmerge | 0.078 | 0.350 |
Number of reflections | 32247 | |
<I/σ(I)> | 10.4 | 3.4 |
Completeness [%] | 99.1 | 96.5 |
Redundancy | 4.3 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 17%-19% PEG 6K, 0.2M NaCl, 0.1M HEPES buffer, pH 7 |