5HO2
Crystal structure of AbnA (open conformation), a GH43 extracellular arabinanase from Geobacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.693, 87.541, 129.234 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.430 - 2.370 |
| R-factor | 0.16853 |
| Rwork | 0.165 |
| R-free | 0.24159 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ho0 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.603 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.078 | 0.350 |
| Number of reflections | 32247 | |
| <I/σ(I)> | 10.4 | 3.4 |
| Completeness [%] | 99.1 | 96.5 |
| Redundancy | 4.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 17%-19% PEG 6K, 0.2M NaCl, 0.1M HEPES buffer, pH 7 |
