5HO1
MamB-CTD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.273 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 38.556, 49.828, 95.882 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.940 - 2.530 |
R-factor | 0.24489 |
Rwork | 0.240 |
R-free | 0.29414 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ho5 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.178 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.940 | 2.650 |
High resolution limit [Å] | 2.530 | 2.530 |
Rmerge | 0.066 | 1.822 |
Number of reflections | 6527 | |
<I/σ(I)> | 16.7 | |
Completeness [%] | 99.1 | |
Redundancy | 6 | |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293.15 | 27% PEG 3350, 0.1 M HEPES pH 7.6, 0.2 M Ammonium sulfate, 5 mM ZnSO4 |