5HNV
Crystal structure of PpkA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 65.753, 78.282, 59.162 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.879 - 1.410 |
| R-factor | 0.184 |
| Rwork | 0.183 |
| R-free | 0.21000 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.490 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.042 | 0.737 |
| Number of reflections | 59128 | |
| <I/σ(I)> | 28.7 | 3.4 |
| Completeness [%] | 99.2 | 95.2 |
| Redundancy | 12.8 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | PEG3350, HEPES, sodium chloride |
