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5HNR

The X-ray structure of octameric human native 5-aminolaevulinic acid dehydratase.

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSRS BEAMLINE PX9.5
Synchrotron siteSRS
BeamlinePX9.5
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date1998-06-01
DetectorMAR scanner 180 mm plate
Wavelength(s)0.87
Spacegroup nameI 4 2 2
Unit cell lengths125.530, 125.530, 200.910
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution44.380 - 2.830
R-factor0.1687
Rwork0.165
R-free0.23785
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1aw5
RMSD bond length0.012
RMSD bond angle1.655
Data reduction softwareMOSFLM
Data scaling softwareSCALA
Refinement softwareREFMAC (5.8.0107)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]54.2003.000
High resolution limit [Å]2.8002.800
Rmerge0.0710.257
Number of reflections18652
<I/σ(I)>62.9
Completeness [%]96.076.7
Redundancy5.93.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.329310 mg/ml protein concentration in 10 milliM Tris pH 7.4, 10 milliM diothiothreitol and 100 microM zinc chloride. 5 microlitres of this were mixed with an equal volume of 0.1 M MES pH range 6.2 - 6.5, 1.0 - 1.6 M ammonium sulphate and 0 - 10 % dioxane.

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