5HNO
The structure of the kdo-capped saccharide binding subunit of the O-12 specific ABC transporter, Wzt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-07 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.03322 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.750, 53.550, 87.470 |
| Unit cell angles | 90.00, 125.77, 90.00 |
Refinement procedure
| Resolution | 35.487 - 1.700 |
| R-factor | 0.1846 |
| Rwork | 0.183 |
| R-free | 0.21550 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.921 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.880 | |
| Number of reflections | 58678 | |
| <I/σ(I)> | 19.93 | 1.87 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 3.7 | |
| CC(1/2) | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M Na HEPESm 0.8 M Na phosphate, 0.8 M K Phosphate |
