5HNL

In-house X-ray single crystal diffraction from protein microcrystals via magnetically oriented microcrystal arrays in gels

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2014-05-07
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	30.530, 56.420, 73.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.746 - 2.424
R-factor	0.1723
Rwork	0.171
R-free	0.18770
RMSD bond length	0.004
RMSD bond angle	0.622
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	56.420
High resolution limit [Å]	2.420
Number of reflections	4958
<I/σ(I)>	6.9
Completeness [%]	96.8
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		313	PEG 4000, Sodium chloride