5HMQ
xylose isomerase-like TIM barrel/4-hydroxyphenylpyruvate dioxygenase fusion protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97936 |
| Spacegroup name | P 63 |
| Unit cell lengths | 262.503, 262.503, 139.644 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.440 - 2.371 |
| R-factor | 0.2054 |
| Rwork | 0.204 |
| R-free | 0.22930 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.744 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.180 | 2.460 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.054 | 0.338 |
| Number of reflections | 220967 | |
| <I/σ(I)> | 18.83 | 2.17 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1 Tris pH 7.5, 0.1 M LiSO4, 25% PEG3350 |
