5HMQ

xylose isomerase-like TIM barrel/4-hydroxyphenylpyruvate dioxygenase fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97936
Spacegroup name	P 63
Unit cell lengths	262.503, 262.503, 139.644
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.440 - 2.371
R-factor	0.2054
Rwork	0.204
R-free	0.22930
RMSD bond length	0.004
RMSD bond angle	0.744
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.180	2.460
High resolution limit [Å]	2.370	2.370
Rmerge	0.054	0.338
Number of reflections	220967
<I/σ(I)>	18.83	2.17
Completeness [%]	100.0	100
Redundancy	2	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1 Tris pH 7.5, 0.1 M LiSO4, 25% PEG3350