5HMQ
xylose isomerase-like TIM barrel/4-hydroxyphenylpyruvate dioxygenase fusion protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97936 |
Spacegroup name | P 63 |
Unit cell lengths | 262.503, 262.503, 139.644 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.440 - 2.371 |
R-factor | 0.2054 |
Rwork | 0.204 |
R-free | 0.22930 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.744 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.180 | 2.460 |
High resolution limit [Å] | 2.370 | 2.370 |
Rmerge | 0.054 | 0.338 |
Number of reflections | 220967 | |
<I/σ(I)> | 18.83 | 2.17 |
Completeness [%] | 100.0 | 100 |
Redundancy | 2 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1 Tris pH 7.5, 0.1 M LiSO4, 25% PEG3350 |