5HMP
Myosin Vc pre-powerstroke state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-08-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.980110 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 103.780, 66.740, 131.260 |
Unit cell angles | 90.00, 104.45, 90.00 |
Refinement procedure
Resolution | 48.500 - 2.397 |
R-factor | 0.206 |
Rwork | 0.205 |
R-free | 0.22650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vom |
RMSD bond length | 0.007 |
RMSD bond angle | 0.998 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.500 | 2.540 |
High resolution limit [Å] | 2.390 | 2.390 |
Number of reflections | 67573 | |
<I/σ(I)> | 13.47 | 1.85 |
Completeness [%] | 98.3 | 96.4 |
Redundancy | 3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG 8000, MES, DMSO |