5HMO
myosin X motor activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.980110 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 69.810, 69.810, 236.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.500 - 3.493 |
| R-factor | 0.3938 |
| Rwork | 0.391 |
| R-free | 0.43710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2lw9 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.869 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.370 | 3.700 |
| High resolution limit [Å] | 3.490 | 3.490 |
| Number of reflections | 7979 | |
| <I/σ(I)> | 15.74 | 2.02 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 13.7 | 2.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | PEG 3350, Ammonium citrate, dioxane |
