5HML
Crystal Structure of T5 D15 Protein Co-crystallized with Metal Ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-10-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.380, 58.380, 59.720 |
| Unit cell angles | 66.53, 79.48, 73.71 |
Refinement procedure
| Resolution | 54.600 - 1.482 |
| R-factor | 0.1433 |
| Rwork | 0.141 |
| R-free | 0.19580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ut5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.324 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 54.602 | 30.861 | 1.560 |
| High resolution limit [Å] | 1.482 | 4.690 | 1.482 |
| Rmerge | 0.029 | 0.491 | |
| Rmeas | 0.069 | ||
| Rpim | 0.039 | 0.019 | 0.341 |
| Total number of observations | 264840 | 8696 | 38322 |
| Number of reflections | 87441 | ||
| <I/σ(I)> | 11.4 | 36.5 | 2.2 |
| Completeness [%] | 93.7 | 96.1 | 92.6 |
| Redundancy | 3 | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 280 | Bis-Tris propane, PEG 3350, MgCl2, NaI |
