5HM7

The Intracellular domain of Butyrophilin 3A1 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 1 21 1
Unit cell lengths	37.430, 145.840, 44.910
Unit cell angles	90.00, 105.57, 90.00

Refinement procedure

Resolution	72.920 - 1.930
R-factor	0.198
Rwork	0.197
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n7i
RMSD bond length	0.011
RMSD bond angle	1.491
Data reduction software	iMOSFLM
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.920	2.020
High resolution limit [Å]	1.930	1.930
Number of reflections	34081
<I/σ(I)>	5.63	1.52
Completeness [%]	98.7	99.7
Redundancy	17.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	298	22% PEG 3350, 0.2M Magnesium chloride, 0.1M HEPES 7.0