5HM7
The Intracellular domain of Butyrophilin 3A1 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.430, 145.840, 44.910 |
| Unit cell angles | 90.00, 105.57, 90.00 |
Refinement procedure
| Resolution | 72.920 - 1.930 |
| R-factor | 0.198 |
| Rwork | 0.197 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n7i |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.491 |
| Data reduction software | iMOSFLM |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.920 | 2.020 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 34081 | |
| <I/σ(I)> | 5.63 | 1.52 |
| Completeness [%] | 98.7 | 99.7 |
| Redundancy | 17.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 22% PEG 3350, 0.2M Magnesium chloride, 0.1M HEPES 7.0 |
