5HM6
N-terminal domain of BfmR from Acinetobacter baumannii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 65 |
Unit cell lengths | 51.800, 51.800, 197.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.860 - 2.000 |
R-factor | 0.20241 |
Rwork | 0.201 |
R-free | 0.22700 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3nhz |
RMSD bond length | 0.009 |
RMSD bond angle | 1.428 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.101 | 0.617 |
Number of reflections | 20243 | |
<I/σ(I)> | 20 | 2.1 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 7 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.1 M Bis-Tris propane pH 8.0 and 20% (v/v) MeOH |