5HLJ

Crystal Structure of Major Envelope Protein VP24 from White Spot Syndrome Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	I 21 3
Unit cell lengths	139.735, 139.735, 139.735
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.246 - 2.406
R-factor	0.173
Rwork	0.171
R-free	0.19260
Starting model (for MR)	SAD
RMSD bond length	0.008
RMSD bond angle	1.125
Data reduction software	SCALEPACK
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.074	0.043
Number of reflections	17758
<I/σ(I)>	10.5
Completeness [%]	99.9	98.8	100
Redundancy	20.6	18	20.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	0.1M Tris-HCl, pH 8.5, 2.75 M NH4AC