5HLG
Structure of reduced AbfR bound to DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9735 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 150.325, 290.504, 52.584 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.000 |
R-factor | 0.22756 |
Rwork | 0.226 |
R-free | 0.25675 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hbl |
RMSD bond length | 0.008 |
RMSD bond angle | 1.256 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 47129 | |
<I/σ(I)> | 24 | |
Completeness [%] | 99.3 | |
Redundancy | 14.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100mM sodium cacodylate, 100mM NaCl, 50mM MgCl2, 12% PEG 5000 |