5HLG
Structure of reduced AbfR bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9735 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 150.325, 290.504, 52.584 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.22756 |
| Rwork | 0.226 |
| R-free | 0.25675 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hbl |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.256 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 47129 | |
| <I/σ(I)> | 24 | |
| Completeness [%] | 99.3 | |
| Redundancy | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100mM sodium cacodylate, 100mM NaCl, 50mM MgCl2, 12% PEG 5000 |
