5HL7

The crystal structure of the large ribosomal subunit of Staphylococcus aureus in complex with lefamulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.971
Spacegroup name	P 65 2 2
Unit cell lengths	282.112, 282.112, 875.343
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.930 - 3.550
R-factor	0.1888
Rwork	0.187
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wce
RMSD bond length	0.010
RMSD bond angle	1.610
Data scaling software	SCALEPACK
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.610
High resolution limit [Å]	3.550	9.610	3.550
Rmerge	0.209	0.116	0.994
Rmeas	0.213	0.121
Rpim	0.055	0.029	0.300
Total number of observations	2606786
Number of reflections	236087
<I/σ(I)>	5
Completeness [%]	95.7	96.9	94.6
Redundancy	11	13.7	9.2
CC(1/2)		0.990	0.626

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1:2 MPD:EtOH 10mM Hepes pH=7.6 10mM Mg2Cl 60mM NH4Cl 15mM KCl