5HL7
The crystal structure of the large ribosomal subunit of Staphylococcus aureus in complex with lefamulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.971 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 282.112, 282.112, 875.343 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.930 - 3.550 |
R-factor | 0.1888 |
Rwork | 0.187 |
R-free | 0.22660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wce |
RMSD bond length | 0.010 |
RMSD bond angle | 1.610 |
Data scaling software | SCALEPACK |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.610 |
High resolution limit [Å] | 3.550 | 9.610 | 3.550 |
Rmerge | 0.209 | 0.116 | 0.994 |
Rmeas | 0.213 | 0.121 | |
Rpim | 0.055 | 0.029 | 0.300 |
Total number of observations | 2606786 | ||
Number of reflections | 236087 | ||
<I/σ(I)> | 5 | ||
Completeness [%] | 95.7 | 96.9 | 94.6 |
Redundancy | 11 | 13.7 | 9.2 |
CC(1/2) | 0.990 | 0.626 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1:2 MPD:EtOH 10mM Hepes pH=7.6 10mM Mg2Cl 60mM NH4Cl 15mM KCl |