5HKP
Crystal structure of mouse Tankyrase/human TRF1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.995, 100.069, 75.951 |
| Unit cell angles | 90.00, 107.54, 90.00 |
Refinement procedure
| Resolution | 79.000 - 2.200 |
| R-factor | 0.1999 |
| Rwork | 0.198 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3utm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.229 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 79.000 | 50.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 5.040 | 2.200 |
| Rmerge | 0.111 | 0.094 | 0.397 |
| Number of reflections | 48080 | ||
| <I/σ(I)> | 7.5 | ||
| Completeness [%] | 98.7 | 99.1 | 97.7 |
| Redundancy | 3.1 | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.035M Calcium Chloride dihydrate 0.07M MES monohydrate pH6.0 0.03M Tris pH8.5 31.5% v/v Polyethylene glycol 200 6% v/v Ethanol |
