5HKH
Crystal structure of Ufm1 in complex with UBA5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2014-12-12 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 82.656, 82.656, 62.027 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.877 - 2.550 |
| R-factor | 0.1984 |
| Rwork | 0.193 |
| R-free | 0.24760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wxs |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.258 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.880 |
| High resolution limit [Å] | 2.550 |
| Number of reflections | 8280 |
| <I/σ(I)> | 41.5 |
| Completeness [%] | 100.0 |
| Redundancy | 19.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium acetate, 0.01 M magnesium acetate, 30% polyethylene glycol 8000, 0.05 M sodium cacodylate, pH 8.0 |
