5HKD
Bacterial sodium channel neck 7G mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2014-03-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0332 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 137.100, 137.990, 173.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.800 |
| R-factor | 0.27467 |
| Rwork | 0.273 |
| R-free | 0.31198 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lto |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.894 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.600 | |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.660 | |
| Number of reflections | 18097 | |
| <I/σ(I)> | 6.22 | |
| Completeness [%] | 97.4 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 277 | 30% PEG400, 20 mM MES, pH 5.8 |
