5HK7
Bacterial sodium channel pore, 2.95 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2014-03-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.999 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 153.510, 160.520, 166.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.950 |
| R-factor | 0.20618 |
| Rwork | 0.205 |
| R-free | 0.23612 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lto |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.173 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.260 | |
| High resolution limit [Å] | 2.950 | 2.950 |
| Number of reflections | 42643 | |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 99.2 | |
| Redundancy | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 27% PEG300, 100 mM sodium acetate, pH 5.5 |
