5HK0

Crystal structure of M. tuberculosis MazF-mt3 (Rv1991c) in complex with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-05-08
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5
Spacegroup name	P 32
Unit cell lengths	68.338, 68.338, 88.364
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.173 - 2.250
R-factor	0.2156
Rwork	0.212
R-free	0.25680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	B. subtilis MazF
RMSD bond length	0.002
RMSD bond angle	0.721
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.290
High resolution limit [Å]	2.250	6.100	2.250
Rmerge	0.054	0.023	0.430
Total number of observations	61859
Number of reflections	21890
<I/σ(I)>	12.4
Completeness [%]	99.6	98.8	95.9
Redundancy	2.8	2.9	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	298	0.8 M Ammonium sulfate, 0.1 M sodium citrate