5HJL
Crystal structure of class I tagatose 1,6-bisphosphate aldolase LacD from Streptococcus porcinus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.7101 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.660, 93.750, 88.110 |
Unit cell angles | 90.00, 120.58, 90.00 |
Refinement procedure
Resolution | 46.870 - 3.000 |
Rwork | 0.174 |
R-free | 0.22920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3iv3 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.914 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.880 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.296 | 1.130 |
Number of reflections | 14194 | |
<I/σ(I)> | 5.9 | 2.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.5 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Small crystals grow in 25% PEG 3350, 0.1M bistris pH 5.5, 0.2 M Ammonium Sulfate. The crystal used for data collection was obtained after micro seeding in the same condition. |