5HJJ
Crystal Structure of Pyrococcus abyssi Trm5a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | Cu FINE FOCUS |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-06 |
Detector | OXFORD ONYX CCD |
Wavelength(s) | 1.5 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.832, 54.324, 129.015 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.559 - 2.000 |
R-factor | 0.1991 |
Rwork | 0.197 |
R-free | 0.24510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hjk |
RMSD bond length | 0.004 |
RMSD bond angle | 0.886 |
Data reduction software | CrysalisPro |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.800 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.136 | 0.740 |
Number of reflections | 25714 | |
<I/σ(I)> | 7.9 | 5.1 |
Completeness [%] | 99.5 | 98.7 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 10-15% PEG 3350, 100 mM HEPES, pH7.5, 100 mM Ca(OAc)2 and 100 mM KCl |