5HJF

The apo form of Dps4 from Nostoc punctiforme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.984002
Spacegroup name	P 63
Unit cell lengths	101.884, 101.884, 146.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.107 - 1.590
R-factor	0.1308
Rwork	0.130
R-free	0.14620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.170
Data reduction software	XDS
Data scaling software	Aimless (0.2.14)
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.750	48.750	1.640
High resolution limit [Å]	1.590	6.150	1.590
Rmerge	0.067	0.054	0.507
Total number of observations	1174206	20298	113526
Number of reflections	115112
<I/σ(I)>	19.6	48.4	3.8
Completeness [%]	99.7	99.8	98.5
Redundancy	10.2	9.9	10.2
CC(1/2)	0.999	0.998	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	25% SOKALAN HP 66, 0.1 M HEPES pH 7.0 and 0.2 M NaOAc