5HJ2

Integrin alpha2beta1 I-domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-04
Detector	PSI PILATUS 6M
Wavelength(s)	.9791
Spacegroup name	P 43 21 2
Unit cell lengths	144.066, 144.066, 130.004
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	101.870 - 2.153
R-factor	0.2354
Rwork	0.234
R-free	0.28350
RMSD bond length	0.010
RMSD bond angle	1.251
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	150.000
High resolution limit [Å]	2.150
Number of reflections	74386
<I/σ(I)>	11.6
Completeness [%]	99.3
Redundancy	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	PEG 8000, HEPES, calcium chloride, glycerol