5HJ2
Integrin alpha2beta1 I-domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-04 |
Detector | PSI PILATUS 6M |
Wavelength(s) | .9791 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 144.066, 144.066, 130.004 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 101.870 - 2.153 |
R-factor | 0.2354 |
Rwork | 0.234 |
R-free | 0.28350 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.251 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 150.000 |
High resolution limit [Å] | 2.150 |
Number of reflections | 74386 |
<I/σ(I)> | 11.6 |
Completeness [%] | 99.3 |
Redundancy | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 278 | PEG 8000, HEPES, calcium chloride, glycerol |