5HJ0

Crystal Structure of Mis18 'Yippee-like' Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.2825
Spacegroup name	P 31 1 2
Unit cell lengths	121.069, 121.069, 73.211
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.630 - 2.640
R-factor	0.22176
Rwork	0.220
R-free	0.26030
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.753
Data reduction software	xia2
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	4.000
High resolution limit [Å]	3.900	3.900
Rmerge	0.095	0.103
Number of reflections	5669
<I/σ(I)>	15.3	3.7
Completeness [%]	97.2	93.9
Redundancy	9.9	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Diffraction quality crystals were obtained using well buffer containing 0.2 M Ammonium chloride/formate/acetate/phosphate and 20% PEG 3350 (with the measured pH of the solution in the range of 6.2 to 8). 15-20 mg/ml protein sample was mixed with the well buffer in a 1:1 ratio.
1	VAPOR DIFFUSION, SITTING DROP		291	Diffraction quality crystals were obtained using well buffer containing 0.2 M Ammonium chloride/formate/acetate/phosphate and 20% PEG 3350 (with the measured pH of the solution in the range of 6.2 to 8). 15-20 mg/ml protein sample was mixed with the well buffer in a 1:1 ratio.