5HIZ

The structure of PEDV NSP9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97910
Spacegroup name	P 64
Unit cell lengths	73.870, 73.870, 91.553
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.900
Rwork	0.272
R-free	0.33380
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.061	0.702
Number of reflections	6415
<I/σ(I)>	36.4	0.061
Completeness [%]	99.4	100
Redundancy	11.2	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	294	0.3 M Tris, 13%(v/v) tert-Butano