5HIU
Structure of the TSC2 N-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.730, 78.100, 153.750 |
| Unit cell angles | 89.90, 89.85, 78.94 |
Refinement procedure
| Resolution | 41.900 - 2.500 |
| R-factor | 0.22577 |
| Rwork | 0.224 |
| R-free | 0.26323 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.226 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.900 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.035 | |
| Number of reflections | 83768 | |
| <I/σ(I)> | 21.8 | |
| Completeness [%] | 96.6 | 95.8 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 100 mM Tris (pH 7.0), 14 % PEG 6000, 15 % Glycerol, 200 mM MgCl2 |
