5HHZ
Crystal structure of maize cytokinin oxidase/dehydrogenase 4 (ZmCKO4) in complex with 6-(3-methylpyrrol-1-yl)-9H-purine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-27 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 80.066, 80.066, 203.213 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.000 |
R-factor | 0.1977 |
Rwork | 0.197 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o95 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.990 |
Data reduction software | XDS (Jun 17, 2015) |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.460 | 2.120 |
High resolution limit [Å] | 2.000 | 2.000 |
Total number of observations | 326446 | |
Number of reflections | 44421 | |
<I/σ(I)> | 22.58 | 2.94 |
Completeness [%] | 97.1 | 97.1 |
Redundancy | 7.35 | 7.07 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES pH 7,5, 50% MPD, infiltrated for 1h with 2 mM 6-(3-methylpyrrol-1-yl)-9H-purine (MPP) in DMSO and 5% glycerol |