5HHN
Crystal Structure of HLA-A*0201 in complex with M1-F5L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.773, 80.202, 57.445 |
| Unit cell angles | 90.00, 114.18, 90.00 |
Refinement procedure
| Resolution | 43.870 - 2.030 |
| R-factor | 0.1732 |
| Rwork | 0.171 |
| R-free | 0.21490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gso |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.570 | 2.140 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.118 | 0.699 |
| Number of reflections | 28609 | |
| <I/σ(I)> | 10.6 | 2 |
| Completeness [%] | 99.4 | 96.8 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 18-22% PEG 3350, Na citrate pH 6.5, 0.1M MgCl2 |
