5HHL

Reverse transcriptase domain of group II intron maturase from Eubacterium rectale in P21 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	74.830, 119.860, 161.470
Unit cell angles	90.00, 92.08, 90.00

Refinement procedure

Resolution	49.143 - 2.100
R-factor	0.2027
Rwork	0.201
R-free	0.23770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hhj
RMSD bond length	0.001
RMSD bond angle	0.424
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2254: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	49.143
High resolution limit [Å]	2.100
Number of reflections	165153
<I/σ(I)>	8.12
Completeness [%]	100.0
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291.15	100 mM MES/imidazole pH 6.5, 20 mM sodium formate, 20 mM NH4OAc, 20 mM trisodium citrate, 20 mM sodium potassium L-tartrate, 20 mM sodium oxamate, 10 % PEG 8000, 20 % EG