5HHG

Mouse importin alpha: Dengue 2 NS5 C-terminal NLS peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-20
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	79.250, 88.960, 100.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.320 - 2.200
R-factor	0.1781
Rwork	0.176
R-free	0.21420
RMSD bond length	0.006
RMSD bond angle	0.978
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_1839)

Data quality characteristics

	Overall
Low resolution limit [Å]	31.320
High resolution limit [Å]	2.200
Number of reflections	36643
<I/σ(I)>	23.5
Completeness [%]	99.8
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	296	1.5M Ammonium Sulfate, 0.1M Sodium HEPES