5HHE
Human Beclin 1 coiled-coil domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-07 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 58.200, 71.575, 58.446 |
Unit cell angles | 90.00, 112.55, 90.00 |
Refinement procedure
Resolution | 40.150 - 1.460 |
R-factor | 0.1733 |
Rwork | 0.172 |
R-free | 0.20810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q8t |
RMSD bond length | 0.007 |
RMSD bond angle | 0.704 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.150 | 1.570 |
High resolution limit [Å] | 1.460 | 1.460 |
Number of reflections | 38596 | |
<I/σ(I)> | 15.91 | 2.02 |
Completeness [%] | 99.7 | 97.9 |
Redundancy | 7.5 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.5 | 293 | 39% 2-methyl-2, 4-pentadiol, 9% PEG400 and 100 mM Tris buffer, pH 7.5. |