5HGZ
Crystal structure of human Naa60 in complex with acetyl-CoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.287, 57.365, 68.818 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.814 - 1.383 |
R-factor | 0.1829 |
Rwork | 0.182 |
R-free | 0.19180 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.529 |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.420 |
High resolution limit [Å] | 1.383 | 1.383 |
Number of reflections | 43682 | |
<I/σ(I)> | 21.5 | 2 |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 6.9 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 4 | 293.15 | 10mM Tris pH 8.0, 75mM NaCl, 0.5% glycerol, 3%(v/v) Tacsimate pH4.0 (Hampton Research) and 7.5%(w/v) polyethylene glycol 3350 (PEG 3350) |